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SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule." This equation is: Tm = 2(A + T) + 4(G + C) Where
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Solved 1. Primer design Use the "2+4 rule" to calculate the | Chegg.com
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Calculating the melting temperature of PCR primers
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How to calculate GC content of DNA if melting temperature of DNA is known - YouTube
SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
Calculating the melting temperature of PCR primers
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